Project 1: Novel Mechanisms for Seizure Mitigation and Neuroprotection

Project 1: Novel Mechanisms for Seizure Mitigation and Neuroprotection

Mice hippocampal neurons and neuronal synchronized Ca2+ oscillations
Mice hippocampal neurons stained with MAP2 (green, soma, axons, dendritic processes), GFAP (red, glia) and NucBlue (nuclei). TETS experimental timeline ad  changes in neuronal network synchronized Ca2+ oscillation patterns with TETS exposure..

The disruption of electrical and Ca2+ signaling events contributes to the induction and severity of seizures as well as promotes brain damage. A main objective of Project 1 is to develop in vitro / ex vivo models, or models of isolated tissue, by using high-content, rapid throughput technology to detect these disruptions. Rapid throughput technology allows  many samples to be processed at once, increasing efficiency. A second objective is to apply these methods to investigate new and existing therapeutic strategies for mitigating the induction, progression and severity of seizures induced by TETS and DFP-intoxication. Leading therapies already in existence will be screened and, along with Cores A and B, new therapeutic targets that delay or mitigate seizure activity and neurological damage produced by the chemical threat agents will be discovered. Findings are then tested by Projects 2 and 3.